Pervanadate Stimulates Amylase Release and Protein Tyrosine Phosphorylation of Paxillin and p125FAK in Differentiated AR4-2J Pancreatic Acinar Cells
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منابع مشابه
Regulation of neutrophil adhesion by pituitary growth hormone accompanies tyrosine phosphorylation of Jak2, p125FAK, and paxillin.
Neutrophil adhesion is fundamentally important during the onset of inflammatory responses. The adhesion signaling pathways control neutrophil arrest and extravasation and influence neutrophil shape and function at sites of inflammation. In the present study the intracellular signaling pathways for the adhesion of human neutrophils by pituitary growth hormone (GH) were examined. Pituitary GH tri...
متن کاملCalcium metabolism and amylase release in rat parotid acinar cells.
1. A method is described for the isolation of rat parotid acinar cells by controlled digestion of the gland with trypsin followed by collagenase. As judged by Trypan Blue exclusion, electron microscopy, water, electrolyte and ATP concentrations and release of amylase and lactate dehydrogenase, the cells are morphologically and functionally intact. 2. A method was developed for perifusion of aci...
متن کاملCharacterization of putrescine- and spermidine-transport systems of a rat pancreatic acinar tumoral cell line (AR4-2J).
Polyamines are polycationic molecules essential for cell growth and differentiation. Recent work has focused on cell polyamine-transport systems as a way to regulate intracellular polyamine levels. In this study, we demonstrate the presence of two different active transporters for putrescine and spermidine in a rat tumoral cell line (AR4-2J). The first has a Km of 3.1 microM and a Vmax of 3.7 p...
متن کاملRegulation of CCK-induced amylase release by PKC- in rat pancreatic acinar cells
Li, Chenwei, Xuequn Chen, and John A. Williams. Regulation of CCK-induced amylase release by PKCin rat pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol 287: G764–G771, 2004. First published June 24, 2004; 10.1152/ajpgi.00111.2004.— PKC is known to be activated by pancreatic secretagogues such as CCK and carbachol and to participate along with calcium in amylase release. Four PKC...
متن کاملCaerulein and gastrin(2-17 ds) regulate differently synthesis of secretory enzymes, mRNA levels and cell proliferation in pancreatic acinar cells (AR4-2J).
In order to characterize the biological functions coupled to cholecystokinin (CCK) A and B receptors, the effects of gastrin(2-17 ds) and caerulein were compared. An isolated cell model, the pancreatic acinar cell line AR4-2J, was used and the experiments were carried out in serum-free media. Caerulein was found to evoke no mitogenic effects either alone or in the presence of the CCK antagonist...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1998
ISSN: 0021-9258
DOI: 10.1074/jbc.273.26.16366